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- lapin polyclonal
Réactivité: Borrelia burgdorferi B31
application: Western blot, ELISA
Western blot showing detection of 0.1 µg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa). Lane 3: DbpA, MBP removed by TEV cleavage. Lane 4: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
quantité: 100 ug
Prix: 329.00 USD
fournisseur
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information sur le gène - Borrelia burgdorferi B31 dbpA
- description:decorin-binding protein A
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type de produit
- dbpA anticorps
- dbpA Protéine
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