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- lapin monoclonal (J.924.2)
Réactivité: common tobacco, humain, souris, rat, poisson-zèbre , Caenorhabditis elegans
application: Western blot, immunohistochimie, immunocytochimie, immunoprécipitation, cytométrie en flux, immunohistochemistry - paraffin section
citations: 1
Immunoprecipitation of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
quantité: 100µL
Prix: US 309.00
fournisseur - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c, humain, souris, rat, vaches, poisson-zèbre , drosophile, platanna, Caenorhabditis elegans, equine
application: Western blot - lapin polyclonal
Réactivité: humain, souris, rat, poisson-zèbre , Caenorhabditis elegans
application: Western blot, immunohistochimie, immunocytochimie, immunoprécipitation, immunohistochemistry - paraffin section
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information sur le gène - Caenorhabditis elegans his-71
- description:Histone H3.3 type 1
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type de produit
- his-71 anticorps
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