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- souris monoclonal (KN382/EC1)
Réactivité: Domestic guinea pig, Chinese hamster, humain, souris, vaches, lapin
application: Western blot, immunohistochimie, immunocytochimie, immunoprécipitation, immunohistochemistry - paraffin section
Immunofluorescent analysis of FKBP52 (green) in HeLa cells either left untreated (left panel) or treated with 0.5mM copper sulfate (right panel) for 24 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a FKBP52 monoclonal antibody (Product # MA1-111) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
quantité: 200µg
Prix: US 425.00
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information sur le gène - Chinese hamster Fkbp4
- synonyme:peptidyl-prolyl cis-trans isomerase FKBP4; FK506 binding protein 4, 59kDa
- description:FK506 binding protein 4
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type de produit
- Fkbp4 anticorps
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