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- souris monoclonal (N3H10)
Réactivité: Chinese hamster, humain, souris, rat, vaches, platanna
application: Western blot, immunohistochimie, immunocytochimie, immunoprécipitation, cytométrie en flux, immunoprécipitation de la chromatine, immunohistochemistry - paraffin section, RNA immunoprécipitation
citations: 50
Formalin-fixed, paraffin-embedded human tonsil stained with Ku antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells and lymphocytes.
Immunofluorescent analysis of Ku (green) in HeLa cells either left untreated (left panel) or treated with 50uM etoposide (right panel) for 3 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Ku (p70) monoclonal antibody (Product # MA5-13110) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
Western blot analysis of Ku (p70) was performed by loading 50ug of HepG2 whole cell lysate per well onto a SDS-PAGE gel. Proteins were transferred to a membrane and blocked with 5% nonfat dry milk in PBS-0.1% Tween-20 for 30 minutes. The membrane was probed with a Ku (p70) monoclonal antibody (Product # MA5-13110) at a concentration of 0.5ug/ml overnight at 4°C on a rocking platform, washed in PBS-0.1%Tween-20, and probed with an anti-mouse IgG-HRP secondary antibody for 1 hour. Detection was performed using a chemiluminescent substrate.
quantité: 500µL
Prix: US 309.00
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information sur le gène - Chinese hamster Xrcc6
- synonyme:X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1
- description:X-ray repair cross complementing 6
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- Xrcc6 anticorps
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