Your Filters
produit
- lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA
Western blot of APG8 fusion protein. Anti-APG8 antibody generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of GeneTex's IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-anti-Rabbit IgG MX
quantité: 250.00μg
Prix: 289 USD
fournisseur - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA 
Western blot of APG8 fusion protein. Anti-APG8 antibody generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of Rockland's IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX (code # 611-103-122). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel. Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no reactivity of anti-APG8 with other UBLs. This data indicates that anti-APG8 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.
quantité: 500 ug
Prix: 329.00 USD
fournisseur - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA
Envoyez-moi un e-mail lorsque de nouvelles informations sur les gènes suivants sont disponibles: ATG8
information sur le gène - Saccharomyces cerevisiae S288c ATG8
- synonyme:APG8; AUT7; CVT5
- description:ubiquitin-like protein ATG8
do you know?
- we limit the listing of re-branded antibodies in order to provide our visitors with accurate information and the best experience
- we manually curate antibody information from literature in order to obtain a comprehensive set of well-validated antibodies.
type de produit
- ATG8 anticorps
- ATG8 cDNA
espèce
linkOut
questions et commentaires