Your Filters
produit
- lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA
citations: 1
Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # 611-703-127) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
quantité: 500 ug
Prix: 329.00 USD
fournisseur - souris monoclonal (4F2.F5.G2)
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA, immunohistochimie 
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
quantité: 100 ug
Prix: 329.00 USD
fournisseur - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot - souris monoclonal (4F2.F5.G2)
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA, immunohistochimie - lapin polyclonal
Réactivité: Saccharomyces cerevisiae S288c
application: Western blot, ELISA
Envoyez-moi un e-mail lorsque de nouvelles informations sur les gènes suivants sont disponibles: SMT3
information sur le gène - Saccharomyces cerevisiae S288c SMT3
- description:SUMO family protein SMT3
do you know?
- we limit the listing of re-branded antibodies in order to provide our visitors with accurate information and the best experience
- we manually curate antibody information from literature in order to obtain a comprehensive set of well-validated antibodies.
type de produit
- SMT3 anticorps
- SMT3 cDNA
linkOut
questions et commentaires