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- lapin polyclonal
Réactivité: humain, chimpanzé
application: Western blot, ELISA
Western blot using Rockland's Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
quantité: 100 ug
Prix: 329.00 USD
fournisseur - lapin polyclonal
Réactivité: humain, chimpanzé
application: Western blot - lapin polyclonal
Réactivité: humain, rat, chien, poulet, chimpanzé
application: Western blot, ELISA
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information sur le gène - chimpanzé PRKDC
- synonyme:DNA-dependent protein kinase catalytic subunit
- description:protein kinase, DNA-activated, catalytic polypeptide
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type de produit
- PRKDC anticorps
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