Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8)
Invitrogen
Catalogue: MA5-15177

lapin monoclonal (F.52.8)
Réactivité: Chinese hamster, humain, souris, rat, poisson-zèbre , drosophile
modification: phosphorylé
application: Western blot, immunohistochimie, immunocytochimie, immunohistochemistry - paraffin section
citations: 2

quantité: 200µL
Prix: US 309.00
fournisseur

Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (S.417.1)
Invitrogen
Catalogue: MA5-15182

lapin monoclonal (S.417.1)
Réactivité: Chinese hamster, humain, souris, rat, vaches, Sus Sucidés, poisson-zèbre
modification: phosphorylé
application: Western blot, immunocytochimie, cytométrie en flux

Flow cytometry analysis of phospho-p38 MAPK (pThr180/Tyr182) on Jurkat cells, either left untreated (blue histogram) or treated with anisomycin (green histogram). Cells were harvested, fixed with formaldehyde for 10 minutes at 37ºC, permeabilized with ice-cold methanol at a final concentration of 90% for 30 minutes on ice, and incubated with a phospho-p38 MAPK (pThr180/Tyr182) monoclonal antibody (Product # MA5-15182) or nonspecific rabbit monoclonal antibody control (red histogram) at a 1:25 dilution for 1 hour at room temperature. For flow analysis, a 30-minute incubation with a fluorescently-conjugated anti-rabbit IgG secondary antibody was performed and data were acquired on a flow cytometer.

Western blot analysis of phospho-p38 MAPK (pThr180/Tyr182) was performed by loading 20ug of HEK293T whole cell lysates, either left untreated (UT, left lane) or incubated with alkaline phosphatase (AP, right lane), per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hr at room temperature. The membranes were probed either with a phospho-p38 MAPK (pThr180/Tyr182) monoclonal antibody (Product # MA5-15182) at a dilution of 1:1000 (top blot) or a total p38 MAPK monoclonal antibody (bottom blot) overnight at 4°C, washed in TBST, and probed with a peroxidase-conjugated goat anti-rabbit IgG at a dilution of 1:40,000 for 1 hr at room temp. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Immunofluorescent analysis of phospho-p38 MAPK (pThr180/Tyr182, green) in UV light-treated HeLa cells. Cells were fixed in 4% formaldehyde for 15 minutes at room temperature, rinsed with PBS, and blocked with 5% normal goat serum in 1X PBS containing 0.3% Triton X-100 for 1 hour. Cells were probed with a phospho-p38 MAPK (pThr180/Tyr182) monoclonal antibody (Product # MA5-15182) at a dilution of 1:100 overnight at 4ºC, washed with PBS, and incubated with a fluorescently-conjugated anti-rabbit IgG secondary antibody for at least 1 hour at room temperature. F-actin (red) was stained with fluorescently-conjugated Phalloidin. Staining was visualized by confocal microscopy.
quantité: 200µL
Prix: US 309.00
fournisseur

Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (C.7.8)
Invitrogen
Catalogue: MA5-15218

souris monoclonal (C.7.8)
Réactivité: humain, souris, rat, poisson-zèbre
modification: phosphorylé
application: Western blot, immunocytochimie, immunoprécipitation, cytométrie en flux

Immunofluorescent analysis of Phospho-p38 MAPK pThr180/Tyr182 (green) in HeLa cells either left untreated (left panel) or treated with 100ng/ml TNF-alpha for 15 minutes (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-p38 MAPK pThr180/Tyr182 monoclonal antibody (Product # MA5-15218) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
quantité: 100µL
Prix: US 309.00
fournisseur

Mapk14 (phospho T180/Y182) polyclonal antibody
Abnova
Catalogue: PAB9665

lapin polyclonal
Réactivité: humain, souris, rat, chien, poulet, vaches, poisson-zèbre
application: Western blot, immunohistochimie

quantité: 100 uL
Prix:
fournisseur

Purified anti-p38 MAPK Phospho (Thr180/Tyr182)
BioLegend
Catalogue: 903501

lapin polyclonal (Poly29035)
Réactivité: humain, souris, rat, chien, poulet, vaches, poisson-zèbre
modification: phosphorylé
application: Western blot, ELISA, immunohistochimie, immunoprécipitation

quantité: 100 μl
Prix: 332 USD
fournisseur

Anti-mapk14a Antibody (C-Terminus) LS-C210593
LifeSpan Biosciences
Catalogue: LS-C210593

lapin polyclonal
Réactivité: poisson-zèbre
application: Western blot, ELISA

quantité:
Prix:
fournisseur